The Microbiome and Cancer

The Human Microbiome

Bacteriophage.pngE Coli.pngBacillus Licheniformis.pngXerocomellus Engelii.png

From left to right: A transmission electron micrograph of a bacterial virus (bacteriophage), scanning electron micrograph of E. coli bacteria (artificially colored), bacteria (white) growing after being spread on a dish, edible mushrooms.

 

Below is a list of information found within this section:


 

What are Microbes?

Microbes are extremely small living organisms that include bacteria and fungi. Viruses, while technically not living organisms (they require a living host to reproduce), are also considered microbes. Individual microbes cannot usually be seen with the naked eye; however, large collections of microbial cells can sometimes be seen. For example, bacteria can form visible colonies or ‘streaks’ when spread on gels containing nutrients for them. Additionally, mushrooms are reproductive structures made by some fungi, and we can certainly see the mold colonies that grow on old food. These visible structures are made up of millions of cells.

 

What is the Human Microbiome?

Large and diverse populations of bacteria, viruses, and fungi occupy almost every surface of the human body.1 It is estimated that there are nearly 30 trillion bacterial cells living in or on each human.2 That is about one bacterium for every cell in the human body!2 These microbes are collectively known as the microbiome.31 Exposure to microbes first occurs during birth and is later influenced by environmental factors, such as diet and exposure to antibiotics.31 Due to differences in environment, diet, and behavior, the specific types of microbes that make up the microbiome can vary greatly between individuals.1 It is thought that every person’s microbiome is slightly different.4 In fact, work is underway to investigate the use of microbiomes to identify individuals, much like fingerprints.4

 

The Microbiome in Human Health and Disease

The millions of organisms that make up the human microbiome play an important role in both health and disease. Within the human body, different types of microbes occupy specific body sites and support the functions of these organs; for example, bacteria living in the gut aid in digestion.31 Environmental factors (such as diet, new microbes or antibiotics) can alter the microbiome and lead to a pro-inflammatory state. Inflammation is known to promote cancer (read more about inflammation and cancer here).

Altered gut bacteria have been linked to several diseases, including:

  1. Inflammatory bowel disease
  2. Irritable bowel syndrome
  3. Obesity
  4. Type 2 diabetes3
  5. Cancer

In addition to digestion, microbes are also support immune function, metabolism, and reproduction.3 While microbes play an important role in maintaining human health, they are also involved in the development and progression of some cancers. Notably, there is evidence to suggest that the microbiome can influence patient responses to cancer treatments (see details below). Scientists are now focused on understanding how microbes influence health, disease, and responses to medical treatments. Of note - some cancer treatments are dependent on inflammation and microbiome mediated inflammation can enhance the efficacy of these therapies.

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The Microbiome and Cancer

Scientists are just beginning to understand the link between the human microbiome and cancer development. Initially, epidemiological (population) studies suggested certain microbes played a role in cancer. Theses studies laid the ground work for laboratory experiments, which are ongoing. While these experiments have improved our understanding of microbes in human health and disease, there is still much more to uncover. Below is an overview of the evidence which suggests microbes play a significant role in specific cancers.

Stomach Cancer

Helicobacter pylori (H. pylori), a spiral-shaped bacterium, provides one of the clearest links between a microbe and cancer development. H. pylori infections are common; the Centers for Disease Control and Prevention (CDC) estimates two-thirds of the world population carries the bacterium.5H. pylori infections are spread by contaminated food and mouth-to-mouth contact.5Once inside the host, H. pylori burrows into the mucous layer that lines the stomach.5 This protected area makes it difficult for the host’s immune system to clear the bacterium.5H. pylori infections do not cause illness in most individuals; however, infection is a major risk factor for ulcers and gastric cancer.5Population studies have shown H. pylori-infected individuals are eight times more likely to develop gastric cancer than non-infected individuals.5The exact mechanism by which H. pylori increase the risk for ulcers and cancer is unknown.5There is evidence, however, to suggest that long-term inflammation triggered by H. pylori infection promotes cancer development.5The CDC recommends testing and treatment for H. pylori after removal of early stage gastric cancer and for individuals with a history of ulcers.5

 

Breast Cancer

In 2016 researchers identified populations of microbes living in breast tissue.6 The implications of the breast tissue microbiome are just beginning to be explored.7Interestingly, breast tissue affected by benign and malignant (cancerous) disease was shown to have different populations of bacteria.87 It is unclear if the differences in breast microbiomes helps to cause the development of tumors or is a result of the disease. In the lab, specific bacteria have been linked to tumor progression.87Mice with a predisposition to develop cancer were infected with Helicobacter hepaticus (H. hepaticus).8 Infected mice had increased mammary gland tumor burden and inflammation compared to uninfected mice.8 These results suggest that H. hepaticus can contribute to cancer progression by promoting inflammation.

 

Skin Cancer

The skin microbiome is diverse and differs by anatomical location.7 Experiments done in mice suggest the microbiome can have either protective or harmful roles in cancer development.79 Mice treated with antibiotics (to kill their microbiome) have an increased risk of melanoma skin cancer and shorter average survival times.79 These results suggest the microbiome plays a protective role against development of this cancer type. On the other side, there is also evidence that the protein ‘tails’ (flagella) of some bacteria promote chronic inflammation, leading to tissue damage and ultimately skin cancer.9 In the lab, mice were genetically modified so that they were unable to respond to bacterial flagella.9 It was found that these mice were protected against an artificially induced cancer, which indicates the inflammatory response to gut bacteria can drive the development of cancer.9

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Transmission electron micrograph of a bacterium with a flagellum.

Researchers from the University of Texas MD Anderson Cancer Center in Dr. Jennifer Wargo's laboratory studies recently did sequencing on the gut and oral microbiomes of patients with melanoma. Patients had oral and gut microbiome samples taken, then were treated with anti-PD-1 therapies, and their responses were measured after 12 weeks. What they found was there were no differences in the oral microbiome between responders and non-responders, yet when they looked at the gut microbiome, they found differences between the gut microbiome of responders and non-responders. The responders to the therapy had different bacteria within the gut, which were associated with a response.10

Colorectal Cancer

In healthy individuals, the bacterium Fusobacterium nucleatum (F. nucleatum) is commonly found living in the mouth.11 Several studies, however, have found F. nucleatum in colorectal adenomas and advanced-stage colorectal cancer.11 Recently, it was found that F. nucleatum induces an inflammatory response and activation of cancer promoting genes.11 The activation of these genes was found to increase the rate of proliferation in colorectal cells.11 As described below, this organism is also involved in the development of resistance to colorectal cancer treatments.

Cervical, Anal, and Oral Cancers

Approximately 15% of all human cancers may be attributed to viruses.12 The human papilloma virus (HPV) is responsible nearly all cases of anal and cervical cancer.12These viruses are also responsible for many cases of mouth and throat cancers.12Interestingly, in healthy individuals, papilloma viruses are a common part of the skin and mucosal microbiotas.13 Viruses are also associated to liver cancer and skin cancer. Click here to learn more about viruses and cancer.

The Microbiome and Cancer Treatment

The impact of the human microbiome on cancer treatment is just beginning to be explored. Recent studies have highlighted the importance and potential impact of microbes on disease recovery.147 Interestingly, the microbiome can support the immune system in the fight against cancer.15For example, cyclophosphamide (a drug used to treat leukemia and lymphomas) was found to influence the microbes living in the gut.15These gut microbes responded by promoting the creation of immune cells, which seems to enhance cyclophosphamide efficacy.15As we have seen in the previous sections, microbes have been shown to promote cancer development by inducing inflammation. This inflammatory response can also have a beneficial impact on cancer treatments.14 Some therapies, such as platinum chemotherapy and CpG-oligonucleotide immunotherapy, are dependent on inflammation.14 Mice that were treated with antibiotics (which killed the gut micobiome) did not respond as well to platinum chemotherapy or CpG-oligonucleotide immune therapy compared to mice with intact gut microbes.14 These results suggest that the gut microbiome enhances the effects of therapies that are dependent on inflammation.14

Resistance to cancer treatments has been linked to the presence of specific kinds of bacteria in the gut. Researchers looking at drug resistance in colorectal cancer patients found an increase in Fusobacterium nucleatum in the gut. The bacterium was shown to block death (apoptosis) of the cancer cells and trigger autophagy, a survival tool for the cancer cells.16

Learn more about apoptosis.

How microbes promote immunity and affect cancer development and treatment responses is still being investigated. It is clear, however, that the microbiome can play an important role in both cancer development and treatment responses. Ultimately, researchers hope to identity and harness microbes that fight cancer and develop ways to eliminate those which promote cancer development.7

 

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